A natural bacterial strain Bacillus pumilus 16: Identification and antibiotic resistance evaluation
نویسندگان
چکیده
Microbial biopreparations are actively used to prevent, diagnose, and treat infectious, allergic, tumor, autoimmune diseases in humans animals; stimulate the growth development of plant crops. Natural bacterial strains with valuable technical properties a vital biological resource for developing new rotating already known microbial preparations world market. This study describes natural strain B. pumilus 16, which was isolated from rhizosphere Cichorium. The identified using morphological physiological parameters, biochemical tests, primers Pum-f. Pum-r. Antibiotic sensitivity antagonistic activity against Escherichia coli were determined by diffusion discs delayed antagonism methods, respectively. (like type strains) fermented arabinose, cellobiose, mannitol, mannose, salicin, sucrose, trehalose, gave positive reaction arginine dihydrolase, ONPG, Voges-Proskauer test. It also negative inositol, raffinose, sorbitol, methyl-D-glucoside, inulin, lecithinase. unlike test strains, capable fermenting citrate. Strain 16 highly sensitive cephalexin (37.9±0.7 mm) enrofloxacin (25.7±8.9 mm); ole-andomycin (17.1±1.9 mm), benzylpenicillin (18.5±1.2 monomycin (16.0±0.6 resist-ant oxacillin. By agar blocks method (7.3±1.5 more pronounced E. recorded than wells (5.3±0.6 mm). Due level activity, effective (two did not show an effect). On basis this, can be recommended inclusion preparation composition national economy.
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Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA::katX2 mutant strain
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ژورنال
عنوان ژورنال: Acta Biologica Sibirica
سال: 2021
ISSN: ['2412-1908']
DOI: https://doi.org/10.3897/abs.7.e78412